Question

Which of the following statements is true about size-exclusion chromatography?

a.

During the separation of a mixture of proteins, protein with smallest molecular weight is eluted first

b.

During the separation of a mixture of proteins, protein with largest molecular weight is eluted first

c.

During the separation of a mixture of proteins, protein with largest molecular weight is eluted last

d.

During the separation of a mixture of proteins, protein with largest molecular weight flow around the beads

Answer: (b).During the separation of a mixture of proteins, protein with largest molecular weight is eluted first

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Q. Which of the following statements is true about size-exclusion chromatography?

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