Q. Why must the radiation source for fluorescence spectrometry be more powerful than for absorption spectroscopy?

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Q. Why do fluorescence spectrometers often use double-beam optics?

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Q. For a molecule to absorb IR, why must the molecule's vibrations cause fluctuations in the dipole moment of the molecule?

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Q. Internal conversion is where

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Q. Why phosphorescence measurements are usually made at a low temperature?

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Q. Fluorescence occurs when

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Q. Thin layer chromatography is

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Q. In gas chromatography, the basis for separation of the components of the volatile material is the difference in

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Q. In reverse phase chromatography, the stationary phase is made

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Q. Ion exchange chromatography is based on the

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Q. The general expression for the appearance of a solute in an effluent is (where V is the elution volume of a substance , V0 void volume, kD distribution constant and Vi internal water volume)

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Q. A combination of paper chromatography and electrophoresis involves

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Q. HPLC stands for

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Q. The eluent strength is a measure of

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Q. HPLC methods include

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Q. Column efficiency is measured in terms of number of plates which is

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Q. For a typical adsorbent such as silica gel, the most popular pore diameters are

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Q. An isocratic elution in HPLC is one in which the composition of the solvent

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Q. In reversed phase HPLC, there is a

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Q. Which of the following statements is true for a refractive index detector in HPLC?

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