Question

What is the next step after organic solvent fractionation with dry ice – ethanol – water mixture?

a.

Refrigerated centrifugation at 3000 g for 15 mins

b.

Drying in freezer

c.

Supernatant fractionation with ethanol

d.

Precipitated protein dissolved in 0.01 to 0.05 M cold buffer

Answer: (a).Refrigerated centrifugation at 3000 g for 15 mins

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Q. What is the next step after organic solvent fractionation with dry ice – ethanol – water mixture?

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