Q. The first step in preparation of affinity chromatography column is _____________

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Q. __________ refers to eluting a compound by creating a competition between the bounded enzymes with its suitable complimentary protein in solution.

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Q. Which among these is not a way of non-specific elution?

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Q. Acetylcholine esterase can be purified by using affinity chromatography with the use of _______ as a ligand.

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Q. Acid phosphatase: Mannose:: Alpha amylase: ______________

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Q. The chromatography in which the sample is forced through a column of the stationary phase by pumping a liquid (mobile phase) at high pressure through the column is referred to as ___________

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Q. The time at which specific analyte elute out is termed as _________

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Q. _______________ refers to process of isolation and purification of compounds.

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Q. Which of the following information cannot be obtained by using analytical HPLC?

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Q. _____________ can be achieved using HPLC by utilizing the fact that certain compounds have different migration rates given a particular column and mobile phase.

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Q. __________________ refers to the process of separating or extracting the target compound from other compounds or contaminants.

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Q. The process of determining the unknown concentration of a compound in a known solution by using HPLC is referred to as _______________

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Q. To alter the retention time, which of the following parameters need not be manipulated?

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Q. The gradient separates the analyte mixture as a function of affinity of the analyte for the mobile phase relative to stationary phase.

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Q. In HPLC, the compounds are eluted using a constant mobile phase composition and is referred to as _____________

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Q. In _________ elution, the compounds are eluted by increasing the strength of the organic solvent during HPLC.

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Q. The chromatography technique in which the stationary phase may be solid or liquid is referred to as ________________

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Q. In which chromatographic technique, the solvent velocity and constant flow rate is controlled?

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Q. In FPLC, the sample separates into various zones of sample components and is referred to as ________

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Q. Which characteristic of the enzyme can be used for purifying it by using FPLC?

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