Q. Given an enzyme with Kₘ of 0.25mM, at what substrate concentration will the velocity of the enzyme reach 1/2 of the Vₘₐₓ? (Vₘₐₓ = 100 mmol/s)

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Q. A low Kₘ value points out a low affinity of an enzyme towards its substrate.

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Q. ______________ is defined as an enzyme molecule’s efficiency to convert maximum substrate molecules into product per unit time.

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Q. The equation which is based on reciprocal form of Miachelis Menten equation at constant [Et] level is referred to as ______________

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Q. Which of the following equation is Eadie-Hofstee plot equation?

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Q. Which of the following is termed as catalytic efficiency?

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Q. What does the equation \(V_0=\frac{V_{max} [S]}{K_m+[S]}\) represent?

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Q. What is the ratio of V₀/Vₘₐₓ when [S] = 10Kₘ?

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Q. 4*10⁷: Catalase :: 25: ___________

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Q. ___________ is defined as the enzyme’s binding efficiency with its appropriate substrate through optimized multiple non-covalent interactions.

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Q. Miachelis Menten model is also referred to as “rapid equilibrium model”.

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Q. Which of the following is based on rearrangement of Lineweaver Burk plot equation by multiplication of [S₀] factor?

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Q. What does the following equation represent?

\(\frac{1}{V_0} = \frac{K_m}{V_{max}} \frac{1}{[S]}+\frac{1}{V_{max}}\)

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Q. Which of these is referred to as Kcat?

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Q. Which of the following assumption was not made in rapid equilibrium model?

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Q. Which of these is not required for electrophilic catalysis?

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Q. _____________ involves donation of electrons from nucleophilic active site residues to a substrate forming a covalent adduct transition state intermediate.

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Q. A type of catalysis which involves covalent intermediate transition state formation between the cationic electrophilic group in the enzyme and the electron rich portion of the substrate is referred to as ____________

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Q. Chymotrypsin: Nucleophilic catalysis:: Carboxypeptidase A: __________

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Q. The phenomenon which involves formation of an additional covalent intermediate in a reaction that helps to reduce the energy of later transition states is referred to as ___________

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