Question

Which of the following is not true for spectrofluorometric method?

a.

The intensity of emitted light is used to study enzyme reaction

b.

Trace impurities in enzyme can stop the emitted radiation

c.

It is based on stoke shift

d.

Hydrolases can be assayed and measured by using this method

Answer: (a).The intensity of emitted light is used to study enzyme reaction

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Q. Which of the following is not true for spectrofluorometric method?

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