Q. Kornberg enzyme has both 3’-5’ and 5’-3’ exonuclease activity.

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Q. Name the bacterium from which Taq DNA polymerase derived?

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Q. Name the enzyme which degrades DNA molecule by breaking its phosphodiester bonds?

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Q. Which of the following is NOT an endonuclease?

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Q. Which of the following endonuclease has been purified from Aspergillus oryzae?

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Q. Name the enzyme which degrade dsDNA after recognizing specific nucleotide sequences?

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Q. Which of the following is NOT the property of restriction enzymes?

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Q. Which of the following type of restriction enzyme does not require ATP for cleavage?

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Q. Name the restriction enzyme which recognizes different sequences, but produces same ends?

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Q. Which enzyme join DNA molecules together?

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Q. Circular DNA used for cloning is called___________

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Q. Without origin of replication, DNA cannot be replicated in the cell.

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Q. Which of the following is NOT a cloning vector?

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Q. Cloning vector has the following properties, except one?

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Q. Which of the following is NOT the characteristics of the plasmid?

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Q. Which of the following is TRUE for YAC?

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Q. Which of the following is NOT an example of Lambda phages?

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Q. Name the vector system used for plant transformation?

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Q. Name the first vector used for a mammalian cell?

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Q. Which of the following cloning vector is used for insect and having a DNA genome?

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