Q. Why is a probe labeled?

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Q. In how many ways a DNA can be labeled?

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Q. What is the function of polynucleotide kinase?

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Q. Fluorescent labeling is an important phenomenon in case of hybridization and manipulations in molecular biology.

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Q. Radiolabelling is generally brought about by addition of radioactive phosphorus.

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Q. During electrophoresis denaturation of the double stranded DNA is brought about by __________

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Q. In case of northern blotting the hybridization occurs between two RNA molecules.

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Q. Northern blotting is performed for ____________

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Q. The process by which a probe is used to screen a library is known as ___________

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Q. PCR is a DNA amplifying in vivo method.

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Q. Which of the following is a mismatch?

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Q. Annealing of primer is facilitated by complementary region.

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Q. Primer used for the process of polymerase chain reaction are ______________

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Q. Polymerase used for PCR is extracted from _____________

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Q. How many DNA duplex is obtained from one DNA duplex after 4 cycles of PCR?

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Q. At what temperature do denaturation of DNA double helix takes place?

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Q. At what temperature do annealing of DNA and primer takes place?

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Q. The temperature at which DNA synthesis takes place is 74˚C.

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Q. The type of DNA amplification where the region of DNA amplified lies on either side of a known segment __________

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Q. Primer complementary to regions used in inverse PCR is _____________

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