Q. What are the basic base-specific cleavage sites used in Maxem and Gilbert method?

View solution

Q. Which step is not involved in base-specific cleavage of DNA fragment?

View solution

Q. The process of electrophoresis is the key to sequencing.

View solution

Q. The dideoxy method is also known as _____________

View solution

Q. Which enzyme is used for the replication in case of Sanger’s method of sequencing?

View solution

Q. With respect to Sanger’s enzymatic method of sequencing pick the odd one out.

View solution

Q. The four reaction mixtures that were prepared, contained ____________

View solution

Q. ddNTP acts as the chain radioactive markers.

View solution

Q. Strand separation for DNA sequencing is done to ____________

View solution

Q. The position of band during electrophoresis indicates the dNTP used as a chain terminator in that mixture.

View solution

Q. Automated sequencing is defined as _________

View solution

Q. Automated sequencing uses _____________

View solution

Q. No chain termination takes place in automated sequencing.

View solution

Q. As different fluorescent tags affect the mobility of fragments thus only one type of tag is used.

View solution

Q. With respect to automated sequencing which of the following is true?

View solution

Q. Which of the following feature is required for the high sensitivity of the tags used for autosequencing?

View solution

Q. Automated sequencing is a real time one trial method of sequencing and is highly handy.

View solution

Q. A third generation sequencing can sequence upto ____________nucleotides per day.

View solution

Q. Semiconservative nature of replication of eukaryotic chromosome was first demonstrated by _______________

View solution

Q. Pick the correct pair with respect to primers used in DNA replication.

View solution