Q. Region 1.1 of the σ factor mimics the DNA and is negatively charged.

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Q. RNA polymerase adds the nucleotides de novo. The first nucleotide added by the polymerase is ____________

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Q. The ternary complex is formed by the polymerase, DNA duplex and the nascent RNA.

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Q. After the binding of polymerase to the DNA template the σ factor is ejected.

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Q. How many nucleotides of the growing RNA chain remain base paired with the template DNA?

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Q. The transcription bubble formed for facilitating RNA synthesis is about _____________ nucleotides long.

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Q. The nascent RNA forms a double helix with the non – coding strand.

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Q. How many proof-reading activities does RNA polymerase have?

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Q. The RNA polymerase can remove only the incorrect bases from the nascent RNA.

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Q. The Gre factor enhances the hydrolytic editing function of the polymerase.

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Q. E. coli polymerase adds upto _____________ nucleotides per second.

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Q. The Gre factor is homologous to the eukaryotic factor _____________

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Q. The most common signal for termination of transcription is self complementation.

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Q. The stem of the hairpin loop of RNA consists mostly of _____________

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Q. The hairpin structure generated in the RNA is followed by a stretch of oligonucleotide complementary to the base ____________

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Q. A stretch of which nucleotide in the DNA acts as the termination signal?

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Q. The rho protein has ____________ subunit.

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Q. The rho proteins are ATP independent proteins.

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Q. The rho protein binds to the RNA recognizes a special structure. It consists of ____________ nucleotides.

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Q. The rut sites are rich in ____________ residues.

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