Q. Which enzyme is involved in the synthesis of the DNA over an RNA template?

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Q. For the construction of DNA library what is the range of genomic DNA that has to be cloned into the vector?

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Q. Which of the following enzymes is responsible for the digestion of the RNA strand in the RNA-DNA hybrid?

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Q. cDNA is synthesized from __________

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Q. During storage of DNA fragments for making library, larger fragments are having more chances of getting lost.

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Q. What is the characteristic of the DNA that is used for the construction of library?

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Q. What is the state of phage cloning vector that is used for the construction of library?

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Q. Which enzyme is used to avoid ligation of separate DNA?

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Q. Partial digestion is preferred for the fragmentation of DNA.

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Q. The ordered steps for the construction of library involves __________

i) Vector preparation
ii) Amplification
iii) Ligation and introduction into the host
iv) Isolation of genomic DNA
v) Fragmentation of DNA

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Q. What is the significance of “c” in cDNA library?

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Q. mRNA can be readily separated from lysed eukaryotic cells adding magnetic beads. What is the sequence of this magnetic bead?

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Q. What is the significance of SI nuclease?

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Q. Which of the following is the genetically engineered insulin?

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Q. Genetically manufactured GH is not effective for?

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Q. Active insulin consists of how many polypeptide chains?

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Q. The first successful active insulin was synthesized in __________

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Q. The Insulin A vector does not contain __________

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Q. Pick the odd one out.

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Q. Genetically engineered somatotropin is formed by introducing DNA fragment from the genome to the plasmid.

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