Q. Which polymerase is used in PCR based mutagenesis?

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Q. Which two genes are absent in the E. coli strain CJ236?

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Q. Write down the name of scientist who has discovered the method of site directed mutagenesis?

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Q. Which phage is used in oligonucleotide directed mutagenesis?

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Q. What is the function of Dpm I endonuclease in Tm method of site directed mutagenesis?

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Q. What is the formula for annealing temperature for polymerase chain reaction primer?

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Q. Which of the following properties is improved by site directed mutagenesis?

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Q. In the following compounds which are involved in nucleotide excision repair?

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Q. NHEJ is ubiquitin independent in eukaryotic organisms.

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Q. What are the two fundamental components of NHEJ?

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Q. In SOS repair system cleavage of LexA and UmuD is mediated by ___________

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Q. In eukaryotes the other name of PCNA is __________

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Q. The genes encoding the translesional polymerases are expressed as part of a pathway known as the SOS response.

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Q. In humans which DNA polymerase is involved?

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Q. Which complex in E. coli performs in translesion synthesis?

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Q. After creation of the bubble which Uvr component are recruited?

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Q. Which two Uvr component molecules scan the DNA during nucleotide excision repair?

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Q. What is the main enzyme that plays a major role in formation of thymine dimer?

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Q. Which enzyme is activated during double stranded break?

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Q. Why recombinational repair system is called double strand break repair?

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