Q. Processing of the DNA at the break site occurs at the 3’ end.

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Q. What are the components of the MRX protein?

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Q. What is the source of energy during the action of SPO11?

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Q. What is the cleavage site of RuvC?

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Q. After cleaved by RuvC the resulting ligated recombination products will only be the splice product.

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Q. Which component cleaves the specific DNA strands at the Holliday junction?

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Q. The RuvAB complex specifically recognizes the Holliday junction in an ATP dependent manner.

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Q. What is the joint molecule?

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Q. What is the central protein of homologous recombination?

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Q. If the foreign DNA lacks the Chi site what will happen?

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Q. What is the sequence of the Chi site that is highly found in the E. coli genome?

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Q. The nuclease activity of the RecBCD is prone to digest the bacterial cell wall from foreign DNA.

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Q. How many times the frequency is increased by the Chi sites?

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Q. What is the speed of driving the RecBCD along the DNA?

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Q. What are the two components of RecBCD pathway involved as motor parts?

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Q. What is the function of Rec component?

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Q. What are the helicase components of the RecBCD pathway?

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Q. Chi is the control site of the RecBCD pathway.

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Q. RecBCD pathway is an ATP dependent process.

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Q. RecBCD enzyme has polymerase and transcriptase activity.

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