Q. You design a plasmid with a gene for Histidine synthesis and a fragment containing ARS. You introduce it yeast culture and try to grow then in Histidine deficit media. What will be your observation?

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Q. Deletion of which domain of ARS would give the least replication rate in eukaryotes?

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Q. Which subunit in MCM is not phosphorylated in MCM after initiation is triggered?

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Q. You are given a DNA fragment with a CBD lesion. Which polymerase(s) will you use to replicate this fragment without taking care of the error?

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Q. We know that Beta clamp in bacteria has two subunits and takes the shape of a ring. Which of this will be characteristic of PCNA which is a beta clamp homologue for eukaryotes?

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Q. What is the function of RFC in eukaryotic replication?

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Q. Cyclin-CDK complex (here considering S cyclin) when active is responsible for phosphorylating several proteins and this often marks then for destruction by ubiquitination. What would be its effect on Cdt activity?

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Q. Which of these would you use as a competitive inhibitor for Cdt?

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Q. What is the fate of phosphorylated Cdc 6?

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Q. Pre-replication complex is necessary to initiate replication. It assembles in______________

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Q. Which Cyclin-CDK complex will you find most active in M phase?

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Q. What is the DNA composition of ARS?

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Q. In an experiment, you are working on mitochondrial chromosomes. Which polymerase will ensure accurate replication?

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Q. To your cultured cell lines, you add growth factors like cytokines which act through MAPK cascade pathway. Which transition will it trigger?

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