Q. For performing EMSA what type of gel should be used?

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Q. While performing EMSA, in which situation will you see the band highest?

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Q. Which of these is not true for TFIIH?

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Q. A mediator helps to connect ___________

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Q. Which factor stimulated phosphorylation of CTD of RNA polymerase II?

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Q. The absence of which factor will prevent TFIIH and TFIIE recruitment?

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Q. Which of this is untrue about TBP?

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Q. While in vitro transcription your RNA pol is stalled at a position due to mistreatment. Which factor could you act to help the RNA pol overcome the stalled stage?

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Q. Promoter proximal elements is positioned ________________

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Q. TATA box in eukaryotes would be present in _________________

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Q. TFIIB can bind to a promoter element in the core promoter of eukaryotes. This element is __________

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Q. Which of these is not a promoter element?

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Q. Which would be an appropriate method to detect the core promoter regions in a eukaryotic gene?

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Q. Repressors are active only when they are at the proximity of the RNA polymerase as they directly associate with the pre-initiation complex. State whether this is true or false.

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Q. Which of these class III promoter type resemble class II?

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Q. If you make a chimeric factor with the DNA binding element of an activator and a functional domain of a repressor, how will this factor behave?

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Q. Which of this element is not orientation independent?

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Q. Which of these promoter elements has a high propensity of developing mutation given the eukaryotic gene was inserted in prokaryotes?

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Q. RAP1 has a ___________________

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Q. We know that loss of DNA bases is highly mutagenic. But a loss of DNA bases from which region will not have much affect at all?

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