Q. Which of the following is not true for type 1 restriction endonuclease?

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Q. Which of the following is not true about type II restriction endonuclease?

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Q. Restriction endonucleases can recognize___________

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Q. What does this sequence GC/GGCCGC recognized and cleaves by Not1 Doesn’t agree to?

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Q. Which of the sequence will Dcm methylate?

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Q. In an experiment you introduce your gene of interest in a wild type E. cloli genome using an episome. However, you fail to see the product protein being formed. What could be the possible reason for this?

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Q. Which of the following enzyme air is a neoisoschizomer?

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Q. Which of these restriction enzymes generate 3’ overhang?

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Q. Restriction enzymes cleave the bond by which type of reaction?

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Q. The Mg²⁺ ion in restriction endonuclease is not bound to ________

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Q. In EcoRV the enzyme is a ____________

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Q. When the DNA enzyme interacts with the cognate DNA, there is _______________

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Q. Methylated DNA doesn’t form kink as________________

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Q. A methylated recognition sequence can act as a competitive inhibitor for the restriction endonuclease.

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Q. In which of the following will you expect plasmids to be absent?

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Q. Which of the following is not true about plasmids?

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Q. Which class of plasmids assists in the production of bacteriocins?

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Q. Which of the following is not a part of RTF?

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Q. In an experiment you want to express your gene of interest in a prokaryote through a plasmid. What would be your ideal copy number?

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Q. Pilli forming gene is a must for plasmid transfer. State whether it is true or false.

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