Q. Conjugation can’t take place between_________________

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Q. In an experiment you have met, bio, thr+ and leu + bacterial strain in test tube 1; and met+, bio+, thr and leu bacterial strain in other test tube 2. Which of the following will not comply with your experimental results?

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Q. In case of mixing his+ leu- strain with his- leu+ strain we can see that the auxotroph behaves as prototroph for these two genes. This is because of________________

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Q. Which gene in a plasmid is indispensible for it to participate in conjugation?

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Q. Which of the following can transfer the genes in bacterial nucleoid as well?

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Q. A HFR bacteria mates with an F- plasmid containing bacteria. What will be the type of bacteria after conjugation is complete?

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Q. All the genes in the HFR that is transferred to the recipient cell before breakage of the conjugation channel is incorporated in the recipient genome. State whether it is true or false.

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Q. In and interrupted mating experiment, it is seem that the after 10 mins Aziᴿ gene in recipient, after 20 minuites there is appearance of tonᴿ and after 15 mins the recipient is lac⁺ , but the recipient is almost never his⁺. Where in the genome is the ori of transfer?

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Q. If genes enter the different F- cells from different HFRs in the order as shown-

Azi 8 88

Ton 10 90

Lac 17 3

Gal 24 11

What is the order of the gene where columns are for different HFR and values denote time?

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Q. In an experiment of determining the order of gene in bacteria we use HFR which is gal+, Lac+, Ara+ and His+. If the recipient is negative for all these genes, and we selectively choose Gal + and get the pattern-

100 % recipients are Gal +
30% are His+
55% are Lac +
And only 5% are Ara+

What would be our inference?

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Q. For complete HFR to enter the recipient, how much time should the two bacterial cells be in contact?

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Q. You study the gene incorporation in recipient using HFR and get the following sequences-
P-W-A-K, W-L-I-K, A-K-I-L, W-P-R-T-W-L. What is the order of the gene?

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Q. In an experiment you have a U-Tube with two strains of bacteria in two arms. In one arm the bacteria are Gal+ and His-, in other the strain is Gal- and His+. If the two arms are joined by a membrane that doesn’t allow bacterial cells to pass to opposite site what will you observe when you plate each arm bacterial strain on minimal media?

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Q. State whether it is true or false- Broad scale mapping can be done using transformation?

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Q. If the genes order in bacteria is His gal met. Which of the following genes have the least probability of getting incorporated alone?

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Q. Which of the following species has natural competence?

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Q. Competence can be generated in some strains of non-competent bacteria by __________

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Q. If a bacterium is heterozygous for a gene then will both the genes have an equal probability of incorporation?

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Q. What is the minimum length of DNA fragment in exogenote required for incorporation of the gene in the endogenote?

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Q. If the probability of incorporation of a gene A from a bacterial cell that has lysed in the medium is a. And the probability of incorporation of another gene B from the same bacteria that has lysed is b. What is the probability of one recipient receiving both?

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