Q. In single stranded nick__________

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Q. In case of chicken foot Holliday intermediate, the template strand for synthesis across lesion is___________

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Q. Recombination occurs in ______________

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Q. What is the level of sequence homology required for site specific recombination?

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Q. Which of the following is a must for site-specific recombination reaction?

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Q. Site specific reaction between two circular plasmids could result in__________________

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Q. In recombinase the activated ______________ residue performs the catalytic attack.

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Q. Site-specific recombination can be called an economic process. State whether it is true or false.

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Q. Which one of these is a cis factor in site specific recombination?

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Q. If there is 8 bp sequence flanking oppositely oriented 13 bp symmetry elements like LoxP, what will be the fate of it after recombination?

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Q. Which of the following is an example of site specific recombination?

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Q. If there is a flanking region between two similarly oriented LoxP sequence, what will be its fate on site-specific recombination?

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Q. Integration of the phage into bacterial genome requires__________________

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Q. Excision of the lamda phage genome from bacterial genome requires IHF and _______________

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Q. 5’ |GAAGTTCCTATGC||CGAAGTTCCTATTC||TCTAGAAA||GTATAGGAACTTC 3’
3’ CTTCAAGGATACG||GCTTCAAGGATAAG||AGATCTTT||CATATCCTTGAAG 5’

If the DNA segments are named a, b, c and d respectively; and d is in opposite orientation to a and b, which of the following is dispensable for recombianse action?

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Q. Which of the following is wrong?

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Q. Site specific recombination is not used in__________________

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Q. In mice you place are protein under an inducible promoter and you insert the gene under study in the loxP flanking region between opposite segments. If this tissue is induced which of the following will you see?

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Q. Mendel used ____________ for his experiments.

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Q. Which of the following relationship was not studies by Mendel?

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