Q. During successful purification scheme, this may be expected that the

View solution

Q. In ion-exchange chromatography

View solution

Q. Which of the following may be added to stabilize the protein after yeast cells disruption?

View solution

Q. Gel-filtration chromatography separates on the basis of

View solution

Q. Affinity chromatography deals with the

View solution

Q. A purified protein sample contains 10 μg of protein and has an enzyme activity of 1 m mole of ATP synthesized/sec (1 unit). What is the specific activity of the final purified sample?

View solution

Q. Proteins separation can be carried out on the basis of

View solution

Q. The best way to determine the location of protein in the purification scheme is to measure the

View solution

Q. The conformational changes from the T to the R state is initiated by

View solution

Q. An allosteric activator

View solution

Q. Bisphosphoglycerate (BPG) cannot bind to the oxygenated R state of hemoglobin because

View solution

Q. The Hill coefficient (nH) for myoglobin and hemoglobin are respectively

View solution

Q. When protein binds two ligands in a non-cooperative manner, then the x-intercept of the Scatchard Plot is

View solution

Q. O₂ binding to hemoglobin results in

View solution

Q. In hemoglobin, allosteric effects occur

View solution

Q. A protein that binds two ligands in a non-cooperative manner will show

View solution

Q. Small molecules affect hemoglobin (Hb) by

View solution

Q. A protein that shows infinite cooperative for binding of n ligands will

View solution

Q. The specificity of a ligand binding site on a protein is based on

View solution

Q. What is the ELISA test intended to measure?

View solution