Question

What would happen if serum is omitted from the ELISA, but all other steps remain same?

a.

Anti-human Ig-conjugate would not bind and be washed away

b.

The OD values would be nearly the same as the assay control

c.

Both (a) and (b)

d.

Anti-human Ig-conjugate would bind non-specifically to the ELISA plate

Answer: (c).Both (a) and (b)

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Q. What would happen if serum is omitted from the ELISA, but all other steps remain same?

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