Q. What is the IUPAC code for an A, C, T ambiguity in a nucleic acid sequence?

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Q. There is a single origin for DNA replication in bacteria. How many origins of replication are found in the much larger genomes of eukaryotes such as mammals?

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Q. The melting of 40-45 bp at the oriC site of E. coli requires

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Q. Overwinding or overtightening of DNA during replication is caused and removed by

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Q. How does ethidium bromide interact with DNA?

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Q. Number of times the two DNA strands are interwined in circular DNA and is known as

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Q. The enzyme responsible for breaking the hydrogen bonds, and thus separating the DNA strands during DNA synthesis is

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Q. Automated DNA sequencing uses the chain termination method

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Q. Okazaki fragments occur on the __________ and are bonded together by __________

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Q. The RNA primers used to initiate replication in E. coli

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Q. An enzyme that recognizes foreign DNA, and results in a cut in that DNA is called

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Q. Which of the following is an active site on its single polypeptide chain for DNA Polymerase I?

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Q. Which of the following is not a structural motif in DNA binding proteins?

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Q. DNA with a G-C content of 50% will melt at approximately

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Q. Who's X-ray work aided Watson and Crick in their discovery of the double helix?

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Q. DNA replication in eukaryotes occurs only in

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Q. Which polymerase is active in DNA repairing

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Q. Which will require a higher temperature to denature?

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Q. In an agarose gel, DNA is moving from

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Q. TM refers to the temperature at which

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