Q. Isolation of genomic DNA follows the same principles as that of obtaining plasmid from E. coli. Which of the following is not included in it?

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Q. How many methods are there for obtaining the plasmid DNA from the bacteria?

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Q. Cell lysis is carried out by which substance?

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Q. Chromosomal or genomic DNA is separated by ____________

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Q. Proteins can be removed via treatment by?

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Q. The nucleic acid remaining in the solution can be precipitated by addition of sodium or ammonium acetate and ethanol.

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Q. Gel electrophoresis separates nucleic acid molecules based on ____________

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Q. The charge present on the DNA backbone is negative. The force required to accelerate the molecules towards anode is directly proportional to the number of ____________

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Q. Force is defined as mass per unit acceleration. As the number of phosphate molecules increases, the charge also increases which increases the force required. The acceleration is dependent on the size of the molecules.

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Q. A piece of DNA that is to be separated is crushed and soaked into the buffer. The majority of DNA diffuses, how can it be separated?

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Q. Another method of DNA recovery is termed as freeze-squeeze. The correct statement for it is?

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Q. If further electrophoresis is used for recovery of DNA from gels, the method is termed as ____________

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Q. The DNA is sliced and is placed into the dialysis tube containing buffer. Further electrophoresis is performed. After it, the DNA moves out of the gel but is retained by buffer.

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Q. Often the DNA or RNA needs to be removed from the gel to the solid support. The process can be termed as ____________

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Q. Which of the following steps is not included in the transferring of DNA?

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Q. If the DNA samples are to be analyzed, the membrane should be studied. Which of them is correct for it?

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Q. In the case DNA is transferred from gel to the membrane, it is known as ____________

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Q. Oligonucleotides can’t be referred to ____________

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Q. Oligonucleotides are synthesized chemically. Which of the following step is involved in oligonucleotide synthesis?

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Q. If the known DNA sequences are immobilized onto a solid support, it is called ____________

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