Question

Linkers are often used in cloning. Choose the incorrect statement for linkers.

a.

These are short chemically synthesized molecules that contain a particular restriction enzyme site within the sequence

b.

They are blunt ended molecules

c.

They are ligated to staggered ended insert molecules by T4 DNA ligase

d.

After treatment with enzyme, both the ends of the linker are staggered

Posted under Genetic Engineering

Answer: (c).They are ligated to staggered ended insert molecules by T4 DNA ligase

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Q. Linkers are often used in cloning. Choose the incorrect statement for linkers.

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