Q. Expression of T7 promoter- lac operator hybrid requires ___________

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Q. When lacUV5 control system is used, addition of IPTG _______ the expression of _________

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Q. There are some advantages of expressing protein as a fusion protein. It may enhance stability, folding ______ and ______ formation.

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Q. A short peptide region fused to a protein of interest is known as ___________

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Q. Glutathione-S-Transferase (GST) enzyme is used for the conjunction of Glutathione molecules and is having a protective function in many organisms.

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Q. If it is desirable to express two or more proteins simultaneously, then it is known as ___________

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Q. For co-expression of proteins, it is possible to have several independent sets of promoters and other signals in a single vector.

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Q. A considerable amount of modification can take place in the ______ region of RNA in _______ before translation. It affects the yield of proteins.

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Q. Strong secondary structure in a message may affect ________

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Q. What is the function of T7 lysozyme?

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Q. As the activity of T7 polymerase is reduced by lysosome, what is an effect on the rate of synthesis after induction?

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Q. Proteins at times are not soluble in the cell and form aggregates known as ___________

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Q. It is not possible to solubilise proteins from aggregated mass of proteins.

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Q. Lon protease is used for degradation of foreign proteins in E.coli cells. It is active against which type of proteins?

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Q. Lon mutation can lead to ______ and _______

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Q. Mutation in gale gene or cpsA-E gene cluster is used for ___________

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Q. How many types of reporter gene constructs are there?

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Q. Desired changes are made in the structure of the promoter and is ligated onto reporter gene. The alteration can be known by measuring the activity.

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Q. ______ fluorescent protein should be used for determination of _______ of subcellular components.

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Q. Sequences can be cloned flanking the reporter genes using the transposon tagging approach. The use of reporter genes to identify sequences expressed in this way is called as ___________

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