Question

Which of the following enzymes’ combined action leads to the generation of sticky ends in the plasmid vector?

a.

Alkaline phosphatase and terminal transferase

b.

Exonuclease III and alkaline phosphatase

c.

Bacteriophage lambda exonuclease and terminal transferase

d.

Exonuclease III and terminal transferase

Posted under Biochemistry

Answer: (c).Bacteriophage lambda exonuclease and terminal transferase

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Q. Which of the following enzymes’ combined action leads to the generation of sticky ends in the plasmid vector?

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