Q. Trypsin is used for dissociating the tissue into single cells.

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Q. In the secondary culture, cells are obtained from _______________________

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Q. EDTA binds the ______________ ions.

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Q. HeLa cells are a cell line.

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Q. Under favorable conditions, the protoplasts can grow into a ____________________

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Q. The process of dedifferentiation in cell culture can give rise to ________________________

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Q. Isolation of an organelle in bulk quantity can be accomplished by _____________

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Q. The isotonic buffer solution prevents _________

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Q. Density gradient centrifugation is a type of isopycnic centrifugation.

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Q. Which of the following can be used to study cellular activities?

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Q. Purification of a protein can be measured as an increase in _____________

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Q. Total nitrogen measurement can be used to measure ____________

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Q. Protein’s solubility depends on the relative balance between protein-solvent and protein-protein interactions.

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Q. Which of the following can be used for selective precipitation of proteins?

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Q. In the liquid column chromatography, there are two phases namely ___________________ and ____________________

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Q. Which of the following uses non-compressible matrix and high pressure?

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Q. When the pH of a protein is lowered _________________________

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Q. A protein is neutral at the isoelectric point.

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Q. Which of the following is used as an ion-exchanger resin?

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Q. In ion-exchange chromatography, proteins bound to the resin can be displaced by increasing the _____________________

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