Q. Which enzyme is active at 72° in the polymerase chain reaction?

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Q. The temperature cycles in a polymerase chain reaction are in the order __________________

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Q. Taq polymerase is a ________________________ polymerase.

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Q. Polymerase chain reaction (PCR) was invented by ______________________

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Q. Bacteriophage Lambda is a major cloning vector.

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Q. Recombinant plasmids are added to a bacterial culture that has been pretreated with _________________ ions.

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Q. Which observation was made by Avery, Macleod, and McCarty?

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Q. The first recombinant DNA molecule was synthesized in the year ______________

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Q. Which enzyme is used to join together two different types of DNA molecules?

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Q. EcoR1 exhibits a two-fold rotational symmetry.

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Q. Which of the following enzymes in bacteria are responsible for restricting the growth of viruses?

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Q. Who developed the chemical techniques to synthesize polynucleotides?

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Q. Which of the following is a commonly used label in blotting techniques?

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Q. Which of the following molecules can be analyzed using a northern blot?

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Q. In which of the following techniques, the DNA is labeled using complementary sequences and then analyzed using autoradiography?

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Q. The procedure by which DNA gel is transferred to a membrane is termed as ___________________

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Q. Nucleic acid hybridization is used to identify ________________________

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Q. Which technique can be used to separate ¹⁵N and ¹⁴N?

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Q. Equilibrium centrifugation is also known as ___________________ centrifugation.

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Q. In rate-zonal sedimentation, the nucleic acids are separated base on __________________

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