Q. In DNA sequencing, the fluorescent traces of the DNA sequences are read by a computer program that assigns bases for each peak in a chromatogram.

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Q. The shotgun approach _______ sequences clones from _____ of cloned DNA.

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Q. The shotgun approach does not require knowledge of physical mapping of the clone fragments, but rather a robust computer assembly program to join the pieces of random fragments into a single, whole-genome sequence.

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Q. Despite the multiple coverage, sometimes certain genomic regions remain unsequenced, mainly owing to cloning difficulties.

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Q. The hierarchical genome sequencing approach is ______

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Q. In hierarchical genome sequencing approach, based on the results of _______ mapping _______of the BAC clones on a chromosome can be determined.

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Q. The hierarchical approach is ____ and _____ than the shotgun approach because it involves an initial clone-based physical mapping step.

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Q. The whole genome shotgun approach can produce a draft sequence very rapidly because it is based on the direct sequencing approach.

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Q. The major challenges in genome assembly are sequence errors, contamination by bacterial vectors, and repetitive sequence regions.

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Q. When a sequence is generated from ____ ends of a single clone, the distance between the two opposing fragments of a clone is fixed to ________ meaning that they are always separated by a distance defined by a _____ length (normally 1,000 to 9,000 bases).

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Q. Which of the following is untrue about base calling and assembly programs?

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Q. Which of the following is incorrect?

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Q. Which of the following is incorrect about Phred?

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Q. Which of the following is incorrect about Phrap?

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Q. VecScreen is a primarily aimed for sequence assembly.

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Q. Which of the following is incorrect about EULER?

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Q. TIGR Assembler is a UNIX program from TIGR for assembly of large shotgun sequence fragments.

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Q. Which of the following is incorrect about ARACHNE?

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Q. The genome annotation process involves two steps: gene prediction and functional assignment.

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Q. Which of the following is incorrect regarding gene annotation?

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