Q. Using ddATP, the resulting synthesis creates a set of nested DNA fragments, each one ending at one of the as in the sequence through the substitution of a fluorescent-labeled ddATP.

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Q. The combined mixture of all labeled DNA fragments is electrophoresed to _____ the fragments by______ and the ladder of fragments is scanned for the presence of each of the four labels.

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Q. The sequence can also be verified by making an oligonucleotide primer complementary to the distal part of the readable sequence and using it to obtain the sequence of the complementary strand on the original DNA template.

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Q. When the process is fully automated, a number of priming sites may be used to obtain sequencing results that give optimal separation of bands in each region of the sequence.

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Q. To sequence larger molecules, individual chromosomes are purified and broken into _____ or larger random fragments, which are cloned into vectors designed for large molecules.

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Q. Many levels of clone redundancy may be required to build a consensus map because individual clones can have _______

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Q. Once the correct map has been obtained, unique overlapping clones are chosen for sequencing.

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Q. An alternative method is to sequence all the subclones, produce a computer database of the sequences, and then have the computer assemble the sequences from the overlaps that are found.

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Q. Which of the following is untrue about Shotgun Sequencing?

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Q. Two common goals in sequence analysis are to identify sequences that encode proteins, which determine all cellular metabolisms, and to discover sequences that regulate the expression of genes or other cellular processes.

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Q. cDNA libraries have been prepared that have the same sequences as the mRNA molecules produced by organisms, or else cDNA copies are sequenced directly by RT-PCR (copying of mRNA by reverse transcriptase followed by sequencing of the cDNA copy by the polymerase chain reaction).

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Q. Using cDNA sequence with the __________ it is much simpler to locate protein-encoding sequences in these molecules.

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Q. An important development for computational purposes was the decision by Craig Venter to prepare databases of partial sequences of the expressed genes, called expressed sequence tags or ESTs.

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Q. The translated sequence can then be compared to a database of protein sequences with the hope of finding a strong similarity to a protein of known function, and hence to identify the function of the cloned EST.

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Q. Investigators are encouraged to submit their newly obtained sequences directly to a member of the International Nucleotide Sequence Database Collaboration, such as the NCBI, DDBJ, and EMBL.

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Q. NCBI reviews new entries and updates existing ones, as requested.

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Q. Which of the given statements is incorrect?

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Q. Which of the given statements is untrue?

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Q. Granting agency requires a certain level of accuracy in case of errors. Which of the given statements is untrue regarding it?

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Q. Which of the following is wrong about GenBank DNA Sequence Entry?

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