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36. Which of the following may be added to stabilize the protein after yeast cells disruption?
a. NaCl
b. Protease inhibitor
c. AMP
d. All of these
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Answer: (d).All of these

37. Gel-filtration chromatography separates on the basis of
a. size and shape using porous beads packed in a column
b. size using porous beads packed in a column
c. shape using porous beads packed in a column
d. none of the above
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Answer: (a).size and shape using porous beads packed in a column

38. Affinity chromatography deals with the
a. specific binding of a protein constituents for another molecule
b. protein - protein interaction
c. protein - carbohydrate interaction
d. none of the above
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Answer: (a).specific binding of a protein constituents for another molecule

39. A purified protein sample contains 10 μg of protein and has an enzyme activity of 1 m mole of ATP synthesized/sec (1 unit). What is the specific activity of the final purified sample?
a. 1,000 units/mg
b. 10,000 units/mg.
c. 100,000 units/mg
d. 1,000,000 units/mg
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Answer: (c).100,000 units/mg

40. Proteins separation can be carried out on the basis of
a. net charge
b. solubility in salt solutions
c. size or mass
d. all of these
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Answer: (d).all of these