E-PolyLearning

Welcome to the largest collection of MCQs on the Internet. Access and Discuss these Multiple choice questions for various competitive Exams and Interviews.
41. The best way to determine the location of protein in the purification scheme is to measure the
a. rate of ATP synthesis
b. changes in the refractive index
c. UV absorption
d. mass spectroscopy of the protein
View Answer Report Discuss 50-50!
Answer: (a).rate of ATP synthesis

42. The conformational changes from the T to the R state is initiated by
a. binding of oxygen to the heme
b. movement of the proximal histidine towards the heme
c. movement of the F-helix, which contains the proximal His
d. reorganization of protein-protein contacts between the individual subunits
View Answer Report Discuss 50-50!
Answer: (a).binding of oxygen to the heme

43. An allosteric activator
a. increases the binding affinity
b. decreases the binding affinity
c. stabilizes the R state of the protein
d. both (a) and (c)
View Answer Report Discuss 50-50!
Answer: (d).both (a) and (c)

44. Bisphosphoglycerate (BPG) cannot bind to the oxygenated R state of hemoglobin because
a. it is displaced from the heme by oxygen
b. it is displaced from the heme by movement of the proximal histidine
c. its binding pocket becomes too small to accommodate BPG
d. BPG binds to the R state with the same affinity as the T state
View Answer Report Discuss 50-50!
Answer: (c).its binding pocket becomes too small to accommodate BPG

45. The Hill coefficient (nH) for myoglobin and hemoglobin are respectively
a. 2.8 and 1.0
b. 1.0 and 2.8
c. 1.2 and 4.5
d. 4.5 and 1.2
View Answer Report Discuss 50-50!
Answer: (b).1.0 and 2.8