E-PolyLearning

11. Isolation of pure culture refers to ___________
a. purification of culture
b. introduction of inoculum
c. separation of a single colony
d. to grow microorganisms on a surface
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Answer: (c).separation of a single colony

12. Enrichment media allows the growth of a large number of varied bacterial species.
a. True
b. Flase
c. May be
d. Can't say
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Answer: False

13. Which of the following is not a physical method for selection of pure culture?
a. Heat treatment
b. pH of the media
c. Cell size and motility
d. Use of dilute media
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Answer: (d).Use of dilute media

14. In physical method of selection, endospore-forming bacteria can be obtained by heating the mixed culture to _____________
a. 70 degree C for 10 minutes
b. 80 degree C for 10 minutes
c. 60 degree C for 10 minutes
d. 90 degree C for 10 minutes
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Answer: (b).80 degree C for 10 minutes

15. Vibrio cholerae can grow in a medium with a pH of _______
a. 5.5
b. 7.0
c. 8.5
d. 2.0
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Answer: (c).8.5

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