DNA Sequencing and DNA Replication MCQs

Welcome to our comprehensive collection of Multiple Choice Questions (MCQs) on DNA Sequencing and DNA Replication, a fundamental topic in the field of Molecular Biology. Whether you're preparing for competitive exams, honing your problem-solving skills, or simply looking to enhance your abilities in this field, our DNA Sequencing and DNA Replication MCQs are designed to help you grasp the core concepts and excel in solving problems.

In this section, you'll find a wide range of DNA Sequencing and DNA Replication mcq questions that explore various aspects of DNA Sequencing and DNA Replication problems. Each MCQ is crafted to challenge your understanding of DNA Sequencing and DNA Replication principles, enabling you to refine your problem-solving techniques. Whether you're a student aiming to ace Molecular Biology tests, a job seeker preparing for interviews, or someone simply interested in sharpening their skills, our DNA Sequencing and DNA Replication MCQs are your pathway to success in mastering this essential Molecular Biology topic.

Note: Each of the following question comes with multiple answer choices. Select the most appropriate option and test your understanding of DNA Sequencing and DNA Replication. You can click on an option to test your knowledge before viewing the solution for a MCQ. Happy learning!

So, are you ready to put your DNA Sequencing and DNA Replication knowledge to the test? Let's get started with our carefully curated MCQs!

DNA Sequencing and DNA Replication MCQs | Page 3 of 11

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Q21.
With respect to automated sequencing which of the following is true?

a.

Detected by radioactive tags

b.

Multiple plane sequencing

c.

Horizontal scanning one sequence per lane

d.

Vertical reading software incorporation

Discuss
Answer: (c).Horizontal scanning one sequence per lane
Q22.
Which of the following feature is required for the high sensitivity of the tags used for autosequencing?

a.

Strong absorption at UV spectrum

b.

Maximum emittion at different wavelengths

c.

Different mobility shifts

d.

Induce circularization of DNA fragments

Discuss
Answer: (b).Maximum emittion at different wavelengths
Q23.
Automated sequencing is a real time one trial method of sequencing and is highly handy.

a.

True

b.

False

c.

May be True or False

d.

Can't say

Discuss
Answer: (b).False
Q24.
A third generation sequencing can sequence upto ____________nucleotides per day.

a.

750000

b.

75000

c.

570000

d.

57000

Discuss
Answer: (a).750000
Q25.
Semiconservative nature of replication of eukaryotic chromosome was first demonstrated by _______________

a.

Walter Flemming on root tip cells of Vicia faba

b.

J. Herbert Taylor, Philip Wood and Walter Hughes on root tip cells of Vicia faba

c.

Walter Flemming on root tip cells of Phaseolus vulgaris

d.

J. Herbert Taylor, Philip Wood and Walter Hughes on root tip cells of Phaseolus vulgaris

Discuss
Answer: (b).J. Herbert Taylor, Philip Wood and Walter Hughes on root tip cells of Vicia faba
Q26.
Pick the correct pair with respect to primers used in DNA replication.

a.

RNA primer- for prokaryotes only

b.

DNA primer-for eukaryotes only

c.

DNA primer- for both prokaryotes and eukaryotes

d.

RNA primer- for both prokaryotes and eukaryotes

Discuss
Answer: (d).RNA primer- for both prokaryotes and eukaryotes
Q27.
Which of the following is correctly matched with its subsequent role?

a.

Topoisomerase II- can remove both positive and negative supercoil in the DNA duplex

b.

Polymerase I- larger fragment responsible for exonuclease activity

c.

DnaA protein- responsible for “melting” of the DNA double helix during replication

d.

DnaB protein- attaches to the newly unwounded single strand of DNA to prevent folding of the strand

Discuss
Answer: (c).DnaA protein- responsible for “melting” of the DNA double helix during replication
Q28.
Replication fork is the junction between the two ___________

a.

Unreplicated DNA

b.

Newly synthesized DNA

c.

Newly separated DNA strands and newly synthesized DNA strands

d.

Newly separated DNA strands and the unreplicated DNA

Discuss
Answer: (d).Newly separated DNA strands and the unreplicated DNA
Q29.
Which of the following does not affect DNA replication?

a.

Antiparallel nature of DNA

b.

End specificity of polymerase

c.

SSB protein

d.

Helicase

Discuss
Answer: (c).SSB protein
Q30.
Who was the first person to analyse the process of replication and on which organism?

a.

Arthur Kornberg: E. coli

b.

John Cairns: E. coli

c.

Arthur Kornberg: Bacillus subtilis

d.

John Cairns: Bacillus subtilis

Discuss
Answer: (b).John Cairns: E. coli
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