Q. The procedure in which cell lysis occur because of shock waves which are formed due to collapses occurring in buffered solution containing cells is referred to as _______________

View solution

Q. Homogenization cannot be done by using which one of the following?

View solution

Q. Homogenization utilizing ____________ is one where in manual pressure is created for cell lysis.

View solution

Q. A _________ has long teflon tube about 30-40 cm long which is bulged at the end and has an automated machine for providing the pressure.

View solution

Q. Homogenization performed by passing the cells at high pressure through narrow orifice which can also cause cell rupture is referred to as _______________

View solution

Q. The technique in which the cells are subjected to cold temperature of about -15° C to -25° C is referred to as _____________

View solution

Q. ____________ technique is nothing but a cold or psychrophilic shock.

View solution

Q. Freezing technique is not preferred unless otherwise there is a need.

View solution

Q. Mortar and pestle, french press and homogenizer are widely used to lyse the cells by ultrasonication.

View solution

Q. __________ is a method which is highly used for lab scale procedures than large scale work.

View solution

Q. _________________ is the main contaminant present in the resultant free extract from cells.

View solution

Q. Enzymes can be isolated from cell free extract by which one of the following methods?

View solution

Q. Which of the following methods cannot be used to isolate enzymes from cell free extract?

View solution

Q. Which one if the chemicals is not used to isolate enzymes from cell free extract?

View solution

Q. ___________ is responsible for maintaining biologically native structure of an enzyme and even nucleic acid.

View solution

Q. Increase or decrease in _________ leads to protonation or deprotonation of bases which leads to melting of DNA in turn isolating the enzyme after centrifugation.

View solution

Q. The increase in pH affects the inter H₂ bonds and intramolecular hydrophobic bonds are majorly affected leading to denaturation of DNA.

View solution

Q. Cetyl trimethyl ammonium bromide is a cationic detergent.

View solution

Q. Which of the following chemical is not used to isolate enzymes from cell free extract?

View solution

Q. __________ is a compound which is frequently used for DNA removal.

View solution