Q. His-Tag allows the purification to be done in fewer steps.

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Q. Which of the following is a disadvantage of organic solvent fractionation?

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Q. A preferable technique to perform fractionation is to cool the enzyme solution.

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Q. What is the next step after organic solvent fractionation with dry ice – ethanol – water mixture?

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Q. Ethanol can be removed from protein precipitate by __________

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Q. Enzyme are soluble in aqueous medium.

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Q. Which of these is not a basic amino-acid?

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Q. __________ is an acidic amino acid.

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Q. Pick odd one out.

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Q. When the enzyme solubility is reduced by reducing the water availability, the enzyme-enzyme interaction start to take place and they will aggregate to form precipitates. This phenomenon is called ______________

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Q. Ammonium sulphate is convenient and effective chemical for fractionation by salting out method.

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Q. Which one of the following cannot be used as alternative to ammonium sulphate?

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Q. What does the equation, log⁡S=Kintercept-Ksalt T represent?

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Q. The process in which there is a free flow low molecular weight compounds across the membrane retaining high molecular weight compound is referred to as _____________

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Q. Which of the following factors does not limit the rate of dialysis?

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Q. Which one of the following is not true for membrane selection?

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Q. Pick odd one out.

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Q. The simplest way to remove ammonium sulphate from the system is _____________

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Q. The volume of the solution which is being dialyzed is referred to as diffusate volume.

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Q. What is retentate?

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