Q. Alkaline protease is purified by using ________

View solution

Q. Cation exchange resin is used to purify __________

View solution

Q. Which of the functional groups are not present in cation exchangers?

View solution

Q. The chromatographic method of separating biochemical mixture of compounds, based on highly specific biological interactions is referred to as ______________

View solution

Q. Which of the following is not a highly specific biological interaction to be used in affinity chromatography?

View solution

Q. Which of this is not true while selecting a solid matrix or column during affinity chromatography?

View solution

Q. In affinity chromatography, the matrix should be chemically and mechanically stable.

View solution

Q. The matrix selected during affinity chromatography must possess certain chemical groups.

View solution

Q. The first step in preparation of affinity chromatography column is _____________

View solution

Q. __________ refers to eluting a compound by creating a competition between the bounded enzymes with its suitable complimentary protein in solution.

View solution

Q. Which among these is not a way of non-specific elution?

View solution

Q. Acetylcholine esterase can be purified by using affinity chromatography with the use of _______ as a ligand.

View solution

Q. Acid phosphatase: Mannose:: Alpha amylase: ______________

View solution

Q. The chromatography in which the sample is forced through a column of the stationary phase by pumping a liquid (mobile phase) at high pressure through the column is referred to as ___________

View solution

Q. The time at which specific analyte elute out is termed as _________

View solution

Q. _______________ refers to process of isolation and purification of compounds.

View solution

Q. Which of the following information cannot be obtained by using analytical HPLC?

View solution

Q. _____________ can be achieved using HPLC by utilizing the fact that certain compounds have different migration rates given a particular column and mobile phase.

View solution

Q. __________________ refers to the process of separating or extracting the target compound from other compounds or contaminants.

View solution

Q. The process of determining the unknown concentration of a compound in a known solution by using HPLC is referred to as _______________

View solution