Q. Which of the following equation is true for \(K_m^{app}\), when the substrates are negatively charged?

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Q. Immobilized enzymes observe diffusional resistance at different levels.

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Q. In case of immobilized enzymes, what type of inhibition is represented by the following equation?

\(V=\frac{V_{max} S}{K_m+\frac{K_m [S_0]K_L^s}{K_p K_l^p}}\)

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Q. Immobilization affects the kinetic constants (Kₘ and Vₘₐₓ) of the enzyme.

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Q. __________ is the average ratio of path length between any points within the particle to their absolute distance apart.

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Q. What does the following equation represent?

V=\(\frac{V_{max}}{1+\frac{K_m^{app}}{[S_0]}}\)

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Q. What does the equation, \(D_a = \frac{V_{max}}{K_L [S_b]}\) represent?

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Q. In case of immobilized enzymes, the equation \(V=K_L ([S_0]-[S])=\frac{V_{max} [S]}{K_m+S+\frac{S^2}{K_S}}\) represents ______________ inhibition.

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Q. Within the micro-environment, the same bulk concentration may give two different stable concentrations.

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Q. What does the following diagram represents?

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Q. The following diagram represents ____________ method of immobilization.

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Q. What does the following diagram represent?

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Q. What does the following diagram represent?

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Q. What does the following diagram represent?

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Q. What does the following diagram represent?

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Q. What does the following diagram represent?

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Q. Which concentration profile of substrate molecules approaching the curved surface of immobilized enzymes is shown in the graph below?

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Q. What does the following graph represent?

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Q. What does the following graph predict?

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Q. What does the following graph predicts?

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