Q. The vector and the DNA insert are cut by different enzymes for convenience.

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Q. What is the major difference between cloning vectors and primary vectors?

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Q. Which of the following is the primary use of an expression vector?

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Q. Under the influence of which ion does E. coli takes up plasmid from the environment?

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Q. The process by which every type of transformant can be identified is __________

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Q. Which of the following does not affect the formation of hybrid DNA?

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Q. Which of the following is not termed as hybridization?

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Q. What is a probe?

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Q. Why is a probe labeled?

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Q. In how many ways a DNA can be labeled?

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Q. What is the function of polynucleotide kinase?

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Q. Fluorescent labeling is an important phenomenon in case of hybridization and manipulations in molecular biology.

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Q. Radiolabelling is generally brought about by addition of radioactive phosphorus.

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Q. During electrophoresis denaturation of the double stranded DNA is brought about by __________

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Q. In case of northern blotting the hybridization occurs between two RNA molecules.

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Q. Northern blotting is performed for ____________

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Q. The process by which a probe is used to screen a library is known as ___________

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Q. PCR is a DNA amplifying in vivo method.

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Q. Which of the following is a mismatch?

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Q. Annealing of primer is facilitated by complementary region.

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