Q. From which organism is the enzyme reverse transcriptase isolated?

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Q. How many primers are used in the process of reverse transcriptase amplification?

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Q. The digestion of mRNA during RT – PCR is carried out by the enzyme ____________

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Q. In RT – PCR the enzyme deoxynucleotydil transferase adds poly – G residues in the __________

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Q. Which of the following serves as the first primer in RT – PCR for eukaryotic RNA?

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Q. Reverse transcriptase produces DNA from RNA.

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Q. RAPDs can be used to detect multiple alleles of a marker.

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Q. The inheritance pattern of RAPD is _______________

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Q. RAPDs are much more convenient markers than RFLPs.

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Q. RAPDs cannot be used for PCR amplification.

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Q. Polymorphism in RAPD is observed because ______________

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Q. With respect to RAPD which of the following is false?

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Q. The linear fragment used for inverted PCR has known ends _____________

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Q. The DNA concentration in inverse PCR is kept low.

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Q. With respect to target DNA used in inverted PCR which of the following is not true?

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Q. Primer complementary to regions used in inverse PCR is _____________

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Q. The type of DNA amplification where the region of DNA amplified lies on either side of a known segment __________

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Q. The temperature at which DNA synthesis takes place is 74˚C.

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Q. At what temperature do annealing of DNA and primer takes place?

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Q. At what temperature do denaturation of DNA double helix takes place?

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