Q. The 32P is added at the 3’ end by polynucleotidyl kinase.

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Q. The end labeled fragment is cleaved in how many pieces?

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Q. Restriction digestion is the only process to achieve sequencing by the Maxem and Gilbert method.

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Q. The denatured strands cannot be separated.

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Q. What are the basic base-specific cleavage sites used in Maxem and Gilbert method?

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Q. Which step is not involved in base-specific cleavage of DNA fragment?

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Q. The process of electrophoresis is the key to sequencing.

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Q. The dideoxy method is also known as _____________

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Q. Which enzyme is used for the replication in case of Sanger’s method of sequencing?

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Q. With respect to Sanger’s enzymatic method of sequencing pick the odd one out.

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Q. The four reaction mixtures that were prepared, contained ____________

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Q. ddNTP acts as the chain radioactive markers.

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Q. Strand separation for DNA sequencing is done to ____________

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Q. The position of band during electrophoresis indicates the dNTP used as a chain terminator in that mixture.

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Q. Automated sequencing is defined as _________

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Q. Automated sequencing uses _____________

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Q. No chain termination takes place in automated sequencing.

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Q. As different fluorescent tags affect the mobility of fragments thus only one type of tag is used.

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Q. With respect to automated sequencing which of the following is true?

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Q. Which of the following feature is required for the high sensitivity of the tags used for autosequencing?

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