Q. To facilitate binding of σ⁷⁰ the melting of the template and non – template strands occurs between the positions ____________

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Q. The isomerisation of σ⁷⁰ of bacterial polymerase is an energy dependent process.

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Q. With respect to the formation of a complex between polymerase and DNA template which of the following is correct?

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Q. How many channels are found in the RNA polymerase clamp?

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Q. The channel that allows the exit of the coding strand is known as ____________

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Q. Region 1.1 of the σ factor mimics the DNA and is negatively charged.

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Q. RNA polymerase adds the nucleotides de novo. The first nucleotide added by the polymerase is ____________

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Q. The ternary complex is formed by the polymerase, DNA duplex and the nascent RNA.

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Q. After the binding of polymerase to the DNA template the σ factor is ejected.

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Q. How many nucleotides of the growing RNA chain remain base paired with the template DNA?

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Q. The transcription bubble formed for facilitating RNA synthesis is about _____________ nucleotides long.

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Q. The nascent RNA forms a double helix with the non – coding strand.

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Q. How many proof-reading activities does RNA polymerase have?

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Q. The RNA polymerase can remove only the incorrect bases from the nascent RNA.

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Q. The Gre factor enhances the hydrolytic editing function of the polymerase.

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Q. E. coli polymerase adds upto _____________ nucleotides per second.

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Q. The Gre factor is homologous to the eukaryotic factor _____________

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Q. The most common signal for termination of transcription is self complementation.

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Q. The stem of the hairpin loop of RNA consists mostly of _____________

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Q. The hairpin structure generated in the RNA is followed by a stretch of oligonucleotide complementary to the base ____________

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