Q. Deformylase has a crucial role to play in the formation of mature polypeptide chains in prokaryotes.

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Q. How many translation initiation factors work hand in hand to initiate prokaryotic translation?

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Q. Which of the following is a GTPase?

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Q. Which of the following is not the function of IF3?

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Q. When the large and small subunit of ribosome associate which of the following is not found within the complex?

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Q. Binding of the large subunit to the small subunit triggers a cascade of reactions. Arrange them in order of their occurrence.

i. Release of IF1
ii. Hydrolysis of IF2-GTP
iii. Formation of vacant A site
iv. Reduced affinity of IF2-GDP

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Q. For the correct addition of amino acid to the growing polypeptide chain which of the following does not play any role?

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Q. The events controlling the correct addition of amino acid is controlled by ____________ proteins.

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Q. The auxillary protein required for the elongation of polypeptide is energy independent.

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Q. Aminoacyl tRNA needs an escort to carry them to the ribosome bound mRNA.

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Q. Once the tRNA is aminoacylated, EF-Tu binds to the tRNA at the ____________

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Q. The activation of GTPase activity of the enzyme EF-Tu occurs after its binding to the aminoacylated tRNA.

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Q. The only fidelity testing of the translation is by observing the energy difference involved in the correct and incorrect base pairing.

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Q. How many mechanisms are involved in the maintenance of the fidelity of translation?

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Q. Which part of the large subunit helps in the formation of the peptide bond?

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Q. Which of the following steps is not required for the process of translocation?

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Q. The completion of translocation requires the action of the factor _________________

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Q. The subunit association with the mRNA occurs before tRNA recruitment in the case of both eukaryotes and prokaryotes.

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Q. Eukaryotic mRNA generally codes for a single protein because the eukaryotic translation includes ____________

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Q. The same number of auxiliary proteins is used to drive the initiation process of both prokaryotes and eukaryotes.

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