Q. If we mutate the DNA ligase and observe the length of the replicated strands in different time slots after replication initiation, what will we observe?

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Q. DNA replication in the two strands proceed in opposite direction as they are aligned oppositely with respect to 3’ and 5’ ends
( 5’——————————-3’
3’——————————-5’).

In this context which of the following is true.

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Q. In case of eukaryotes replication initiates at ________

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Q. Here are names of some factors necessary for prokaryotic replication. Which of these or their homologue is unnecessary for eukaryotes?

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Q. You wanted to find out the importance of the DNA polymerases. So, you mutated the polymerases one by one and checked for survival. In which of these mutant cases will you see least survival rate?

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Q. You design a circular ssDNA with a labeled RNA primer (alpha-P³² labeling). You add polymerase epsilon and required enzymes for replication to the fragment. What will you expect to see on autoradiography after gel electrophoresis?

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Q. You design a plasmid with a gene for Histidine synthesis and a fragment containing ARS. You introduce it yeast culture and try to grow then in Histidine deficit media. What will be your observation?

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Q. Deletion of which domain of ARS would give the least replication rate in eukaryotes?

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Q. Which subunit in MCM is not phosphorylated in MCM after initiation is triggered?

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Q. You are given a DNA fragment with a CBD lesion. Which polymerase(s) will you use to replicate this fragment without taking care of the error?

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Q. We know that Beta clamp in bacteria has two subunits and takes the shape of a ring. Which of this will be characteristic of PCNA which is a beta clamp homologue for eukaryotes?

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Q. What is the function of RFC in eukaryotic replication?

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Q. Cyclin-CDK complex (here considering S cyclin) when active is responsible for phosphorylating several proteins and this often marks then for destruction by ubiquitination. What would be its effect on Cdt activity?

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Q. Which of these would you use as a competitive inhibitor for Cdt?

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Q. What is the fate of phosphorylated Cdc 6?

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Q. Pre-replication complex is necessary to initiate replication. It assembles in______________

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Q. Which Cyclin-CDK complex will you find most active in M phase?

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Q. What is the DNA composition of ARS?

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Q. In an experiment, you are working on mitochondrial chromosomes. Which polymerase will ensure accurate replication?

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Q. To your cultured cell lines, you add growth factors like cytokines which act through MAPK cascade pathway. Which transition will it trigger?

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