Q. You transcribe a gene and obtain mRNA. To detect which strand is transcribed you replicate each strand in vitro and use the newly synthesized strand for hybridization with mRNA. If mRNA hybridize with the newly replicated strand from 1st strand then the gene transcribed was in ________

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Q. When sigma subunit dissociates from the RNA polymerase what happens to the DNA binding clamp of the core?

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Q. In an experiment you wrongly added Rifampicin antibiotic to Rifampicin sensitive bacteria. You try to correct your mistake by adding more core subunits (resistant to Rifampicin) to your mixture. What would you expect to see?

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Q. Which is the primary sigma factor in Bacillus subtilis?

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Q. What is the function of 1st subunit of sigma factor in σ⁷⁰ and σ⁴³?

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Q. Loosening the binding between polymerase and non-specific site is important to confer higher specificity. In Bacillus subtilis what is responsible for this action?

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Q. We know that region 2.4 of sigma factor binds DNA at -10box. What is the function of 2.1?

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Q. Which DNA binding domain is seen in region 4.2 of sigma factor?

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Q. Which sigma factor is bound to Rse A?

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Q. You artificially increase the level of σ³² in an E. coli culture. What will you expect to see?

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Q. If a bacteria is facing lack of carbon sources in the media, which sigma factor concentration is expected to increase?

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Q. You want your bacterial culture to grow well so you made an enriched media with all forms of carbohydrates. Which of this carbohydrate should you restore first if you want the culture to keep growing at the same rate?

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Q. In a mero-diploid you have one I- and one I+ Repressor gene in the two lac operons. I+ is wild type while I- is a mutant which produces a repressor that doesn’t bind. What would be the functionality of the diploid?

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Q. Which of these Lac operon mero- diploid will be constitutively turned on? Considering the terms has their usual meaning.

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Q. Which of these combinations will result is am operon that can never produce beta galactosidse?

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Q. Which of the Ars operon regulation site is actually preventing RNA polymerase from binding and producing the Ara genes BAD?

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Q. Which of these Ara genes is a mode of feedback autoregulation?

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Q. In which of this condition is Ara operon functional?

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Q. Which of the Ara promoter regulation is controlled by binding of repressors on the O1 regulating unit?

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Q. Which of these operons is anabolic?

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