Q. Loosening the binding between polymerase and non-specific site is important to confer higher specificity. In Bacillus subtilis what is responsible for this action?

View solution

Q. We know that region 2.4 of sigma factor binds DNA at -10box. What is the function of 2.1?

View solution

Q. Which DNA binding domain is seen in region 4.2 of sigma factor?

View solution

Q. Which sigma factor is bound to Rse A?

View solution

Q. You artificially increase the level of σ³² in an E. coli culture. What will you expect to see?

View solution

Q. If a bacteria is facing lack of carbon sources in the media, which sigma factor concentration is expected to increase?

View solution

Q. You want your bacterial culture to grow well so you made an enriched media with all forms of carbohydrates. Which of this carbohydrate should you restore first if you want the culture to keep growing at the same rate?

View solution

Q. In a mero-diploid you have one I- and one I+ Repressor gene in the two lac operons. I+ is wild type while I- is a mutant which produces a repressor that doesn’t bind. What would be the functionality of the diploid?

View solution

Q. Which of these Lac operon mero- diploid will be constitutively turned on? Considering the terms has their usual meaning.

View solution

Q. Which of these combinations will result is am operon that can never produce beta galactosidse?

View solution

Q. Which of the Ars operon regulation site is actually preventing RNA polymerase from binding and producing the Ara genes BAD?

View solution

Q. Which of these Ara genes is a mode of feedback autoregulation?

View solution

Q. In which of this condition is Ara operon functional?

View solution

Q. Which of the Ara promoter regulation is controlled by binding of repressors on the O1 regulating unit?

View solution

Q. Which of these operons is anabolic?

View solution

Q. Which of these systems give the best mode for turning trp operon off?

View solution

Q. A Lac repressor is a tetramer repressed when bound to the inducer. The trp repressor is a ____________________

View solution

Q. Which of these properties do not agree with trp operon attenuator?

View solution

Q. Which segments of the attenuator together form the repression loop?

View solution

Q. We know that lactose has a positive impact on the activity of the lac operon. Tryptophan’s presence has a ________________

View solution