Q. We know that lactose has a positive impact on the activity of the lac operon. Tryptophan’s presence has a ________________

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Q. If in an experiment you mutate the two trp codons in the attenuator to ala codon what will be the impact on repression?

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Q. Which of these items have a positive impact on PEDCBA transcription?

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Q. When uncharged tRNA concentration is low what will you expect as the activity of tryptophan operon?

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Q. How many stem loop structures play important role in complete system of tryptophan gene regulation?

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Q. Which of this mode of trp regulation is not possible in eukaryotes?

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Q. In which microorganism will you find attenuation by alternate loop formation due to ribosomal stalling?

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Q. Which operon behaves similar to Bacillus subtilis trp operon in terms of attenuation?

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Q. Prokaryotes have 1 RNA polymerase. How many RNA polymerases are there in eukaryotes?

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Q. You isolate the nucleolus from the nucleus and performed a density gradient centrifugation with the extract. Then you check the different for the RNA polymerase activity of the different segment. Which RNA polymerase would you expect to find in abundance?

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Q. Which RNA polymerase can bind CBP?

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Q. What is the sequence of the heptad repeat of C terminal domain?

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Q. You designed an antibody against phosphorylated CTD of RNA polymerase II. Then you use a DNA fragment with lac operon and try to immunoprecipitate the RNA pol II. What will be the expected result?

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Q. If you consider a transcribing RNA pol II with a transcript as small as 30 bases, which serine residue in its CTD will be phosphorylated?

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Q. Which factor phosphorylates the serine residues in RNA pol II CTD?

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Q. In what sequence are the serine residues in RNA pol II phosphorylated?

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Q. In an experiment you add growing concentration of the alpha amanitin. What will be the sequence in which RNA polymerases are affected if at all?

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Q. RPA 2 is one of the larger subunits of RNA polymerase in eukaryotes. It is homologous to the prokaryotic _____________

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Q. How many of eukaryotic RNA polymerase subunits are common for all three polymerases?

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Q. All the RNA polymerases share 5 common subunits, which are present in all the three polymerases and are all alike. State whether the statement is true or false.

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