Q. All the RNA polymerases share 5 common subunits, which are present in all the three polymerases and are all alike. State whether the statement is true or false.

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Q. In a Drosophila polytene chromosome just before molting you add green labelled antibody against unphosphhorylated RNA pol II CTD and red labelled antibody against phosphorylated RNA pol II CTD. These give fluorescence on adding the respective substrates. What will be the colour in gene 74 EF and 75 B?

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Q. Which of this is a cis binding element?

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Q. You wanted to study the assembly of preinitiation complex in eukaryotes. This is by the means of protein-protein interaction. Which of these methods you can’t absolutely put in use there?

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Q. For performing EMSA what type of gel should be used?

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Q. While performing EMSA, in which situation will you see the band highest?

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Q. Which of these is not true for TFIIH?

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Q. A mediator helps to connect ___________

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Q. Which factor stimulated phosphorylation of CTD of RNA polymerase II?

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Q. The absence of which factor will prevent TFIIH and TFIIE recruitment?

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Q. Which of this is untrue about TBP?

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Q. While in vitro transcription your RNA pol is stalled at a position due to mistreatment. Which factor could you act to help the RNA pol overcome the stalled stage?

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Q. Promoter proximal elements is positioned ________________

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Q. TATA box in eukaryotes would be present in _________________

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Q. TFIIB can bind to a promoter element in the core promoter of eukaryotes. This element is __________

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Q. Which of these is not a promoter element?

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Q. Which would be an appropriate method to detect the core promoter regions in a eukaryotic gene?

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Q. Repressors are active only when they are at the proximity of the RNA polymerase as they directly associate with the pre-initiation complex. State whether this is true or false.

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Q. Which of these class III promoter type resemble class II?

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Q. If you make a chimeric factor with the DNA binding element of an activator and a functional domain of a repressor, how will this factor behave?

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