Q. Prokaryotes have 1 RNA polymerase. How many RNA polymerases are there in eukaryotes?

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Q. You isolate the nucleolus from the nucleus and performed a density gradient centrifugation with the extract. Then you check the different for the RNA polymerase activity of the different segment. Which RNA polymerase would you expect to find in abundance?

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Q. Which RNA polymerase can bind CBP?

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Q. What is the sequence of the heptad repeat of C terminal domain?

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Q. You designed an antibody against phosphorylated CTD of RNA polymerase II. Then you use a DNA fragment with lac operon and try to immunoprecipitate the RNA pol II. What will be the expected result?

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Q. If you consider a transcribing RNA pol II with a transcript as small as 30 bases, which serine residue in its CTD will be phosphorylated?

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Q. Which factor phosphorylates the serine residues in RNA pol II CTD?

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Q. In what sequence are the serine residues in RNA pol II phosphorylated?

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Q. In an experiment you add growing concentration of the alpha amanitin. What will be the sequence in which RNA polymerases are affected if at all?

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Q. RPA 2 is one of the larger subunits of RNA polymerase in eukaryotes. It is homologous to the prokaryotic _____________

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Q. How many of eukaryotic RNA polymerase subunits are common for all three polymerases?

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Q. All the RNA polymerases share 5 common subunits, which are present in all the three polymerases and are all alike. State whether the statement is true or false.

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Q. In a Drosophila polytene chromosome just before molting you add green labelled antibody against unphosphhorylated RNA pol II CTD and red labelled antibody against phosphorylated RNA pol II CTD. These give fluorescence on adding the respective substrates. What will be the colour in gene 74 EF and 75 B?

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Q. Which of this is a cis binding element?

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Q. You wanted to study the assembly of preinitiation complex in eukaryotes. This is by the means of protein-protein interaction. Which of these methods you can’t absolutely put in use there?

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Q. For performing EMSA what type of gel should be used?

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Q. While performing EMSA, in which situation will you see the band highest?

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Q. Which of these is not true for TFIIH?

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Q. A mediator helps to connect ___________

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Q. Which factor stimulated phosphorylation of CTD of RNA polymerase II?

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