Question

In an experiment you perform the following- you attach a flag epitope to HDAC, you over express a mutated Mad1-pro gene within the experimental cell, you immunoprecipitate the protein complex using flag epitope and run that on a non-denaturing SDS PAGE and western blot. Now you add antibodies for HDAC, MAD1 and SIN3A stripping after each addition. What will you observe?

a.

Bands at same position every time

b.

3 bands at different positions one at a time

c.

Bands at same position on 2 occasions

d.

No band

Posted under Cytogenetics

Answer: (c).Bands at same position on 2 occasions

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Q. In an experiment you perform the following- you attach a flag epitope to HDAC, you over express a mutated Mad1-pro gene within the experimental cell, you immunoprecipitate the...

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