Q. Which nucleotide is present in the 5’ cap?

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Q. Which is the 1st enzyme in capping?

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Q. The post transcriptional modifications are done after the transcript is completely transcribed. State whether the statement is true or false.

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Q. CBP acts as a(an)_______________

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Q. In an experiment you try to hybridize a DNA single strand with a mature RNA. You observe loops being formed. These loops have ____________

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Q. Which of the following corresponding to the same gene will you expect to be the shortest?

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Q. Splicing concensus sequence is ___________

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Q. In the consensus sequence 5′-AG/GUAAGU–intron–YNCURAC–YnNAG/G-3′ Y could be ______

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Q. In an experiment, you are trying to pull down the site where guanyl transferase binds by co-immunoprecipitation. You run a gel and autoradiograph to see a band denoting GSK-RNA pol II (phosphorylated). What will you see when you perform the same experiment with overexpression of GSK in one setup and overexpression of unlabelled GSK-RNA pol II in the other setup?

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Q. The poly a tail protects the 3’ end from____________

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Q. Which of the following mRNA will not be polyadenylated?

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Q. Which of the box is retained in mature mRNA?

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Q. Which of these sequences will mark a site of polyadenylation?

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Q. Which of the following does not directly interact with DNA?

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Q. CPSF and CStF bind to ____________

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Q. PAP has a processivity of ___________

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Q. Which experimental method can you use to detect 3’ end processing?

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Q. IN case of splicing the attacking group is _________________

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Q. In the case of group 1 RNA, which one of the following can’t attack?

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Q. The 3’- OH end of the branch point A makes the attack in splicing of an intron.

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